Fructose 6 Phosphate Kinase Antibody (N-term)

AMM04616G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
AMM04616G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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Fructose 6 Phosphate Kinase Antibody (N-term)

品牌	Leading Biology	貨號(hào)	AMM04616G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Fructose 6 Phosphate Kinase antibody (N-term).
分子量	85183 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm {ECO:0000255\|HAMAP-Rule:MF_03184}.
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	122-151 aa
靶點(diǎn)	This Fructose 6 Phosphate Kinase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-151 amino acids from the N-terminal region of human Fructose 6 Phosphate Kinase.
亞型	Rabbit Ig
通用名	PFKX
基因ID	5213
UniProt ID	P08237
功能	Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
總結(jié)	Phosphofructokinase catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Only M type PFK isozyme is expressed in mature muscle, while erythrocytes contain both L and M subunits. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7), also known as Tarui disease.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100
別名	ATP-dependent 6-phosphofructokinase, muscle type {ECO:0000255\|HAMAP-Rule:MF_03184}, ATP-PFK {ECO:0000255\|HAMAP-Rule:MF_03184}, PFK-M, 27111 {ECO:0000255\|HAMAP-Rule:MF_03184}, 6-phosphofructokinase type A, Phosphofructo-1-kinase isozyme A, PFK-A, Phosphohexokinase {ECO:0000255\|HAMAP-Rule:MF_03184}, PFKM, PFKX
圖像	Western blot analysis of lysate from human skeletal muscle tissue lysate, using PFKM Antibody （S137）(Cat. AMM04616G). AMM04616G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
說明書	Product Datasheet Protocol
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Fructose 6 Phosphate Kinase Antibody (N-term)