品牌 |
Leading Biology |
貨號 |
AMM04615G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Fructose 6 Phosphate Kinase (PFKM) antibody (C-term).
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分子量 |
85183 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03184}.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
746-776 aa
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靶點(diǎn) |
This Fructose 6 Phosphate Kinase (PFKM) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 746-776 amino acids from the C-terminal region of human Fructose 6 Phosphate Kinase (PFKM).
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亞型 |
Rabbit Ig
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通用名 |
PFKX
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基因ID |
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UniProt ID |
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功能 |
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
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總結(jié) |
Phosphofructokinase catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Only M type PFK isozyme is expressed in mature muscle, while erythrocytes contain both L and M subunits. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7), also known as Tarui disease.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, IF, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
IF~~1:10~50
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別名 |
ATP-dependent 6-phosphofructokinase, muscle type {ECO:0000255|HAMAP-Rule:MF_03184}, ATP-PFK {ECO:0000255|HAMAP-Rule:MF_03184}, PFK-M, 27111 {ECO:0000255|HAMAP-Rule:MF_03184}, 6-phosphofructokinase type A, Phosphofructo-1-kinase isozyme A, PFK-A, Phosphohexokinase {ECO:0000255|HAMAP-Rule:MF_03184}, PFKM, PFKX
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圖像 |
Western blot analysis of PFKM polyclonal antibody (Cat. AMM04615G) in Hela cell line lysate. PFKM(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PFKM Antibody (C-term)(Cat. AMM04615G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKM antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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說明書 |
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數(shù)量 |
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