We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality TRPM5 antibody.

130844 Da

Rabbit

Human, Mouse, Rat

Peptide GSGKKRGKFVKVPS(C), corresponding to amino acid residues 32-45 of mouse TRPM5 (Accession Q9JJH7).????Intracellular, N-terminus.

The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named afterthe first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3.The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM71,2.The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to theirlong N- and C-termini they are also named the long TRP channel family1-3.TRPM4 and TRPM5 are the only TRP channels that are permeable to monovalent cations but not to Ca2+.TRPM4 is expressed as at least two alternative spliced isoforms, TRPM4a and TRPM4b which consist of shorter and longer N-terminal regions, respectively. TRPM4a displays little activity, TRPM4b and TRPM5 appear to be directly activated by an increase in intracellular Ca2+ concentration4,5.TRPM5 is primarily expressed in taste buds and also in stomach, small intestine, uterus and testes2.Regulation of TRPM5 by G-protein-coupled receptors and PLC-mediated IP3 production has been substantiated by the colocalization of TRPM5, of a taste-cell-specific PLC isoform and of receptors for sweet, bitter and amino acid (umami) tastes in taste tissue. It has furthermore been shown that desensitization of TRPM5 following receptor stimulation can be reversed by phosphatidylinositol (4,5)-bisphosphate (PIP2), the substrate of PLC2.Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of the mouse TRPM5. The Anti-TRPM5 antibody (#AG1343) can be used in western blot and immunohistochemical applications. It has been designed to recognize TRPM5 from mouse, rat and human samples.

Affinity purified antibody, Liquid

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

WB

WB~~1:200-1:2000 IHC~~1:100

Transient receptor potential cation channel subfamily M member 5, Long transient receptor potential channel 5, LTrpC-5, LTrpC5, MLSN1- and TRP-related gene 1 protein, Trpm5 {ECO:0000312|MGI:MGI:1861718}

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