總結(jié) |
Adrenoceptors (also called Adrenergic receptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States).Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. The Adrenoceptors are members of the G-protein-coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.The Adrenoceptors are divided into three types: α1, α2 and β adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3. 1,2They are expressed in nearly all peripheral tissues and in the central nervous system.1,2 All α1-Adrenoceptors (α1-ARs) activate phospholipases C and A2.3In addition to mobilizing intracellular calcium, the α1-ARs have also been shown to activate calcium influx via voltage-dependent and independent calcium channels.4 The α1B-Adrenoceptor shows the highest levels in the spleen, kidney, cerebellum, and fetal brain.5α1B Adrenoceptor causes contraction of smooth muscle cells and thereby controls vascular tone, blood pressure, and accelerates the development of atherosclerosis.5 Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of the human α1B- Adrenoceptor. Anti-α1B-Adrenoceptor (extracellular) antibody (#AG1378) can be used in western blot, immunocytochemistry and indirect flow cytometry applications. It has been designed to recognize α1B-Adrenoceptor from human, rat and mouse samples.
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圖像 |
Western blot analysis of α1B-adrenoreceptor in lysates of rat brain (lanes 1and 3), rat kidney (lanes 2 and 4) and GH3 cell line (lanes 5 and 6):
1,2,5. Anti-α1B-Adrenoceptor (extracellular) antibody ( APR11385G), (1:200).
3,4,6. Anti-α1B-Adrenoceptor (extracellular) antibody, preincubated with the control peptide antigen.
Expression of α1B-Adrenoceptor in GH3 cells
Immunocytochemical staining of GH3 cells with Anti-α1B-Adrenoceptor (extracellular) antibody ( APR11385G), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). Nuclear staining of cells using the DNA dye Hoechst 33342 (blue).
Indirect flow cytometry analysis of GH3 living cells:
Black: Unstained cells.
Green: Cells + Anti-α1B-Adrenoceptor (extracellular) antibody ( APR11385G), (10 μg/5x105 cells).
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