PFKP Antibody (C-term)

APR07006G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR07006G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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Tel: 010-56256916
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PFKP Antibody (C-term)

品牌	Leading Biology	貨號(hào)	APR07006G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PFKP antibody (C-term).
分子量	85596 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm {ECO:0000255\|HAMAP-Rule:MF_03184}.
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	754-784 aa
靶點(diǎn)	This PFKP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 754-784 amino acids from the C-terminal region of human PFKP.
亞型	Rabbit Ig
通用名	PFKF
基因ID	5214
UniProt ID	Q01813
功能	Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
總結(jié)	Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:8000 IHC-P~~1:50~100
別名	ATP-dependent 6-phosphofructokinase, platelet type {ECO:0000255\|HAMAP-Rule:MF_03184}, ATP-PFK {ECO:0000255\|HAMAP-Rule:MF_03184}, PFK-P, 27111 {ECO:0000255\|HAMAP-Rule:MF_03184}, 6-phosphofructokinase type C, Phosphofructo-1-kinase isozyme C, PFK-C, Phosphohexokinase {ECO:0000255\|HAMAP-Rule:MF_03184}, PFKP, PFKF
圖像	All lanes : Anti-PFKP Antibody (D769) at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
說(shuō)明書(shū)	Product Datasheet Protocol
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