品牌 |
Leading Biology | 貨號 |
APR07004G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PCK1 antibody (C-term).
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分子量 |
69195 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse, Rat
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免疫原 |
592-622 aa
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靶點(diǎn) |
This PCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 592-622 amino acids from the C-terminal region of human PCK1.
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亞型 |
Rabbit Ig
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通用名 |
PEPCK1
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基因ID |
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UniProt ID |
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功能 |
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
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總結(jié) |
This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, IF, E
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稀釋方法 |
WB~~1:1000
IF~~1:10~50
IHC-P~~1:50~100
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別名 |
Phosphoenolpyruvate carboxykinase, cytosolic [GTP], PEPCK-C, PCK1, PEPCK1
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圖像 |
Western blot analysis of PCK1 Antibody (C-term) (Cat. APR07004G) in rat primary hepatocyte cell line lysates. PCK1 (arrow) was detected using the purified Pab. PCK1 Antibody (C-term) (Cat. APR07004G) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PCK1 antibody detected the PCK1 protein (arrow). Confocal immunofluorescent analysis of PCK1 Antibody (C-term)( APR07004G) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). |
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說明書 |
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數(shù)量 |
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