PCK2 Antibody (N-term)

APR08952G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR08952G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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PCK2 Antibody (N-term)

品牌	Leading Biology	貨號(hào)	APR08952G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PCK2 antibody (N-term).
分子量	70730 Da
細(xì)胞定位	Antigen Cellular Localization: Mitochondrion.
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	24-54 aa
靶點(diǎn)	This PCK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human PCK2.
亞型	Rabbit Ig
通用名	PEPCK2
基因ID	5106
UniProt ID	Q16822
功能	Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
總結(jié)	This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, IF, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100 IF~~1:10~50
別名	Phosphoenolpyruvate carboxykinase [GTP], mitochondrial, PEPCK-M, PCK2, PEPCK2
圖像	Western blot analysis of anti-PCK2 Antibody (N-term) (Cat. APR08952G) in HepG2 cell line lysates (35ug/lane). PCK2 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. Western blot analysis of PCK2 (arrow) using rabbit polyclonal PCK2 Antibody (Q39) (Cat. APR08952G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCK2 gene.
說(shuō)明書	Product Datasheet Protocol
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