品牌 |
Leading Biology | 貨號(hào) |
APR07175G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality SHMT1 antibody (N-term).
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分子量 |
53083 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
19-47 aa
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靶點(diǎn) |
This SHMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-47 amino acids from the N-terminal region of human SHMT1.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Interconversion of serine and glycine.
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總結(jié) |
This gene encodes the cellular form of serinehydroxymethyltransferase, a pyridoxal phosphate-containing enzymethat catalyzes the reversible conversion of serine andtetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate.This reaction provides one carbon units for synthesis ofmethionine, thymidylate, and purines in the cytoplasm. This gene islocated within the Smith-Magenis syndrome region on chromosome 17.Alternative splicing of this gene results in 2 transcript variantsencoding 2 different isoforms. Additional transcript variants havebeen described, but their biological validity has not beendetermined.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
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別名 |
Serine hydroxymethyltransferase, cytosolic, SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT1
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圖像 |
Anti-SHMT1 Antibody (N-term) at 1:1000 dilution + LNCaP whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST. SHMT1 Antibody (N-term) (Cat. APR07175G) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the SHMT1 antibody detected the SHMT1 protein (arrow). SHMT1 Antibody (N-term) (APR07175G)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SHMT1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
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說(shuō)明書(shū) |
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數(shù)量 |
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