ALOX12 Antibody (C-term)

APR10855G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR10855G		100 μl / ￥5450

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安諾倫（北京）生物科技有限公司

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ALOX12 Antibody (C-term)

品牌	Leading Biology	貨號	APR10855G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ALOX12 antibody (C-term).
分子量	75694 Da
細胞定位	Antigen Cellular Localization: Cytoplasm, cytosol. Membrane. Note=Membrane association is stimulated by EGF
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	618-650 aa
靶點	This ALOX12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 618-650 amino acids from the C-terminal region of human ALOX12.
亞型	Rabbit Ig
通用名	12LO, LOG12
基因ID	239
UniProt ID	P18054
功能	Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)- hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation.
總結(jié)	Oxygenase and 14,15-leukotriene A4 synthase activity.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, IF, FC, E
稀釋方法	IF~~1:100 WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50
別名	Arachidonate 12-lipoxygenase, 12S-type, 12S-LOX, 12S-lipoxygenase, Lipoxin synthase 12-LO, 332-, Platelet-type lipoxygenase 12, ALOX12, 12LO, LOG12
圖像	Immunofluorescent analysis of A549 cells, using ALOX12 Antibody (C-term) (Cat. APR10855G). APR10855G was diluted at 1：100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor? 554 (red) conjugated Phalloidin (red). Western blot analysis of ALOX12 Antibody (C-term) (Cat. APR10855G) in K562 cell line lysates (35ug/lane). ALOX12 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX12 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
說明書	Product Datasheet Protocol
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