CYP51A1 Antibody (Center)

APR10867G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR10867G		100 μl / ￥5450

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安諾倫（北京）生物科技有限公司

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CYP51A1 Antibody (Center)

品牌	Leading Biology	貨號(hào)	APR10867G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CYP51A1 antibody (Center).
分子量	56806 Da
細(xì)胞定位	Antigen Cellular Localization: Endoplasmic reticulum membrane. Microsome membrane
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	250-279 aa
靶點(diǎn)	This CYP51A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human CYP51A1.
亞型	Rabbit Ig
通用名	CYP51
基因ID	1595
UniProt ID	Q16850
功能	Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.
總結(jié)	CYP51A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	IHC-P, FC, WB, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50
別名	Lanosterol 14-alpha demethylase, LDM, CYPLI, Cytochrome P450 51A1, Cytochrome P450-14DM, Cytochrome P45014DM, Cytochrome P450LI, Sterol 14-alpha demethylase, CYP51A1, CYP51
圖像	Western blot analysis of lysate from LNCap cell line, using CYP51A1 Antibody (Center)( APR10867G). APR10867G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug. Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CYP51A1 Antibody (Center) (Cat. APR10867G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. CYP51A1 Antibody (Center) (Cat. APR10867G) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
說明書	Product Datasheet Protocol
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