EMX1 Antibody (C-term)

APR14109G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR14109G		100 μl / ￥5450

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安諾倫（北京）生物科技有限公司

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EMX1 Antibody (C-term)

品牌	Leading Biology	貨號(hào)	APR14109G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality EMX1 antibody (C-term).
分子量	28039 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus {ECO:0000255\|PROSITE- ProRule:PRU00108, ECO:0000269\|PubMed:20887964}. Cytoplasm. Note=Might be shuttling between the nucleus and the cytoplasm
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
免疫原	228-257 aa
靶點(diǎn)	This EMX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-257 amino acids from the C-terminal region of human EMX1.
亞型	Rabbit Ig
基因ID	2016
UniProt ID	Q04741
功能	Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
總結(jié)	EMX1 is a transcription factor, which in cooperation with EMX2. It acts to generate the boundary between the roof and archipallium in the developing brain. It may function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, FC, IF, E
稀釋方法	WB~~1:1000 FC~~1:10~50 IF~~1:10~50
別名	Homeobox protein EMX1, Empty spiracles homolog 1, Empty spiracles-like protein 1, EMX1
圖像	EMX1 Antibody (C-term) (Cat. APR14109G) western blot analysis in U251 and Neuro-2a cell line mouse cerebellum tissue lysates (35ug/lane).This demonstrates the EMX1 antibody detected the EMX1 protein (arrow). EMX1 Antibody (C-term) (Cat. APR14109G) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Fluorescent confocal image of Hela cell stained with EMX1 Antibody (C-term)( APR14109G).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EMX1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). EMX1 immunoreactivity is localized to Nucleus significantly.
說明書	Product Datasheet Protocol
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