TIEG Antibody (N-term)

APR06006G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR06006G		100 μl / ￥4950

大規(guī)格詢價

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品牌	Leading Biology	貨號	APR06006G
產品分類	Polyclonal Antibodies	研究領域
產品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality TIEG Antibody (N-term).
分子量	52555 Da
細胞定位	Antigen Cellular Localization: Nucleus {ECO:0000250\|UniProtKB:O89091}.
宿主	Rabbit
種屬反應性	Human
免疫原	13-40 aa
靶點	This TIEG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-40 amino acids from the N-terminal region of human TIEG.
亞型	Rabbit Ig
通用名	TIEG, TIEG1
基因ID	7071
UniProt ID	Q13118
功能	Transcriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis (By similarity). May play a role in the cell cycle regulation.
總結	TIEG is a transcriptional repressor involved in the regulation of cell growth. TIEG binds to the consensus sequence 5'-GGTGTG-3'and inhibits cell growth.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50
圖像	All lanes : Anti-TIEG Antibody (N-term) at 1:2000 dilution Lane 1: 293T whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: human kidney lysates Lane 4: human lung lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of anti-TIEG Antibody (N-term) (Cat.APR06006G) pre-incubated with and without blocking peptide in CEM cell line lysate. TIEG(N-term)(arrow) was detected using the purified Pab . Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with TIEG antibody (N-term) (Cat.APR06006G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
說明書	Product Datasheet Protocol
數量

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