NRAS Antibody (C-term)

APR06005G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR06005G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

Tel: 400-965-8633
Tel: 010-56256916
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NRAS Antibody (C-term)

品牌	Leading Biology	貨號(hào)	APR06005G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality NRAS Antibody (C-term).
分子量	21229 Da
細(xì)胞定位	Antigen Cellular Localization: Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note=Shuttles between the plasma membrane and the Golgi apparatus
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	147-179 aa
靶點(diǎn)	This NRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-179 amino acids from the C-terminal region of human NRAS.
亞型	Rabbit Ig
通用名	HRAS1
基因ID	4893
UniProt ID	P01111
功能	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
總結(jié)	NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, FC, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50
圖像	Anti-NRAS Antibody (C-term) at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST. NRAS Antibody (C-term) (Cat. APR06005G) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NRAS antibody detected the NRAS protein (arrow). Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with NRAS antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
說明書	Product Datasheet Protocol
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