ARHE Antibody (Center)

APR06007G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR06007G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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ARHE Antibody (Center)

品牌	Leading Biology	貨號	APR06007G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ARHE Antibody (Center).
分子量	27368 Da
細(xì)胞定位	Antigen Cellular Localization: Golgi apparatus membrane; Peripheral membrane protein
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse, Rat
免疫原	133-165 aa
靶點	This ARHE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-165 amino acids from the Central region of human ARHE.
亞型	Rabbit Ig
通用名	ARHE, RHO8, RHOE
基因ID	390
UniProt ID	P61587
功能	Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins.
總結(jié)	Members of the Rho family of Ras-related GTPases, such as ARHE, regulate the organization of the actin cytoskeleton in response to extracellular growth factors. Like Ras (MIM 190020), Rho family members appear to cycle between an inactive GDP-bound form and an active GTP-bound form. Three major regulators of Rho activity have been identified: RhoGDIs, which interact with the GDP-bound Rho proteins to keep them in a resting complex (see MIM 601925); GEFs, which promote GDP/GTP exchange leading to activation of Rho proteins (see MIM 601855); and GAPs, which stimulate GTP hydrolysis and return the activated Rho protein to its inactive form (see MIM 602680) (Nobes et al., 1998 [PubMed 9531558]).
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50
圖像	All lanes : Anti-ARHE Antibody (Center) at 1:2000 dilution Lane 1: mouse brain lysate Lane 2: rat brain lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of ARHE Antibody (Center)(Cat. 3APR06007G) in mouse brain tissue lysates (35ug/lane). ARHE (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ARHE antibody (Center) (Cat.APR06007G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
說明書	Product Datasheet Protocol
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