品牌 |
Leading Biology |
貨號(hào) |
AMM05829G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
|
產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality DARPP-32 antibody.
|
分子量 |
32 KDa
|
宿主 |
Rabbit
|
種屬反應(yīng)性 |
Rat
|
靶點(diǎn) |
Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH.
|
基因ID |
|
UniProt ID |
|
總結(jié) |
DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac? (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).
|
形式 |
Prepared from rabbit serum by affinity purification via chromatography on an affinity column prepared with the N-terminal peptide used as antigen.
|
儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
|
應(yīng)用 |
WB, IHC
|
稀釋方法 |
WB~~ 1:1000
IHC~~ 1:250
|
別名 |
Protein phosphatase 1 regulatory subunit 1B, DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, Ppp1r1b
|
圖像 |
Westernblot of rat hippocampal lysate showing specific immunolabeling of the ~32k DARPP protein.
Immunochemical staining of cultured CD1 mouse striatal cells showing specific labeling ofDARPP-32 in green and Tuj1 in red. Cells and photo courtesy of QBMCellScience.
|
說明書 |
|
數(shù)量 |
|