品牌 |
Leading Biology | 貨號 |
AMM08730G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality (Mouse) Scx antibody (N-term).
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分子量 |
M=22;H=22 KDa
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細胞定位 |
Antigen Cellular Localization:
Nucleus {ECO:0000255|PROSITE- ProRule:PRU00981}
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宿主 |
Rabbit
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種屬反應性 |
Mouse
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免疫原 |
69-102 aa
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靶點 |
This mouse Scx antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 69-102 amino acids from the N-terminal region of mouse Scx.
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基因ID |
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UniProt ID |
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功能 |
Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.
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總結(jié) |
Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
WB, IHC-P
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稀釋方法 |
IHC-P~~1:25
WB~~1:1000
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別名 |
Basic helix-loop-helix transcription factor scleraxis, Scx
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圖像 |
AMM08730G staining Scx in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-Scx Antibody (N-term) at 1:1000 dilution Lane 1: mouse lung lysates Lane 2: mouse liver lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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說明書 |
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數(shù)量 |
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