品牌 |
Leading Biology | 貨號 |
APC00098G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality EPOR antibody (C-term).
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分子量 |
H=55 KDa
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細胞定位 |
Antigen Cellular Localization:
Cell membrane; Single-pass type I membrane protein
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
470-504 aa
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靶點 |
This EPOR antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 470-504 amino acids from the C-terminal region of human EPOR.
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基因ID |
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UniProt ID |
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功能 |
Receptor for erythropoietin. Mediates erythropoietin- induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.
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總結(jié) |
Receptor for erythropoietin. Mediates erythropoietin- induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, FC
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稀釋方法 |
WB~~1:1000
FC~~1:25
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別名 |
Erythropoietin receptor, EPO-R, EPOR
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圖像 |
All lanes : Anti-EPOR Antibody (C-term)(APC00098G) at 1/1000 dilution Lane 1: human kidney lysates Lane 2: HL-60 whole cell lysates Lane 3: Jurkat whole cell lysates Lane 4: K562 whole cell lysates Lane 5: HepG2 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 62 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Flow cytometric analysis of K562 cells using EPOR Antibody (C-term)(green, APC00098G) compared to an isotype control of rabbit IgG(blue). APC00098G was diluted at 1:25 dilution. An Alexa Fluor? 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody. |
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說明書 |
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數(shù)量 |
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