品牌 |
Leading Biology | 貨號(hào) |
AMM08751G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality AGR2 antibody (N-term).
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分子量 |
H=20;M=20;Z=20 KDa
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細(xì)胞定位 |
Antigen Cellular Localization:
Secreted. Endoplasmic reticulum
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
13-42 aa
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靶點(diǎn) |
This Anterior Gradient 2 (AGR2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human Anterior Gradient 2 (AGR2).
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通用名 |
AG2
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基因ID |
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UniProt ID |
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功能 |
Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
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總結(jié) |
Anterior gradient 2 (AGR2) is known as a cancer cell marker specifically up-regulated in response to depletion of serum and oxygen. AGR2 has been identified as a tumor marker in primary and secondary cancer lesions, and as a marker for detection of circulating tumor cells (CTCs). Elevated levels of AGR2 are known to increase the metastatic potential of cancer cells, but conditions leading to increased expression of AGR2 are not well understood.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, IF
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
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別名 |
AGR2; AG2; Anterior gradient protein 2 homolog; HPC8; Secreted cement gland protein XAG-2 homolog
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圖像 |
All lanes : Anti-AGR2 Antibody (N-term)(AMM08751G) at 1/1000 dilution Lane 1: HT-29 whole cell lysates Lane 2: T47D whole cell lysates Lane 3: MCF-7 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody (N-term) (Cat. AMM08751G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Fluorescent confocal image of A549 cell stained with AGR2 Antibody (N-term)( AMM08751G).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AGR2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly. |
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說(shuō)明書 |
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數(shù)量 |
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