品牌 |
Leading Biology |
貨號(hào) |
APR17815G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality MME antibody (Center).
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分子量 |
H=86;M=86;Rat=86 KDa
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細(xì)胞定位 |
Antigen Cellular Localization:
Cell membrane; Single-pass type II membrane protein
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse, Rat
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免疫原 |
97-126 aa
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靶點(diǎn) |
This MME antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 492-525 amino acids from the Central region of human MME.
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通用名 |
EPN
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基因ID |
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UniProt ID |
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功能 |
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675). Involved in the degradation of atrial natriuretic factor (ANF) (PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).
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總結(jié) |
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV- inducible elastase activity toward skin preelastic and elastic fibers.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, E
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稀釋方法 |
WB~~1:1000
IHC~~1:25
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別名 |
Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, CALLA, Enkephalinase, Neutral endopeptidase 2411, NEP, Neutral endopeptidase, Skin fibroblast elastase, SFE, CD10, MME, EPN
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圖像 |
All lanes : Anti-MME Antibody (Center) at 1:1000 dilution
Lane 1: LNCaP whole cell lysate
Lane 2: mouse kidney lysate
Lane 3: rat kidney lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 86 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of lysates from LNCaP cell line,mouse kidney and rat kidney tissue lysate(from left to right),using MME Antibody (Center)(Cat. APR17815G). APR17815G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using MME Antibody (Center)( APR17815G). APR17815G was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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說明書 |
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數(shù)量 |
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