品牌 |
Leading Biology | 貨號 |
APG01463G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ACAT1 antibody (C-term).
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分子量 |
45200 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Mitochondrion.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse
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免疫原 |
296-329 aa
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靶點(diǎn) |
This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-329 amino acids from the C-terminal region of human ACAT1.
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亞型 |
Rabbit Ig
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通用名 |
ACAT, MAT
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基因ID |
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UniProt ID |
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功能 |
Plays a major role in ketone body metabolism.
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總結(jié) |
ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
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別名 |
Acetyl-CoA acetyltransferase, mitochondrial, Acetoacetyl-CoA thiolase, T2, ACAT1, ACAT, MAT
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圖像 |
All lanes : Anti-ACAT1 Antibody (C-term) at 1:2000 dilution Lane 1: SW620 whole cell lysates Lane 2: mouse liver lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 45. 2 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-ACAT1 Antibody (C-term) at 1:1000 dilution Lane 1: SK-BR-3 whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 45. 2 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of anti-ACAT1 Pab (Cat. APG01463G) in SK-BR-3 cell line lysates (35ug/lane).ACAT1 (arrow) was detected using the purified Pab. |
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說明書 |
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數(shù)量 |
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