IDH1 Antibody (Center)

APR07923G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR07923G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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IDH1 Antibody (Center)

品牌	Leading Biology	貨號	APR07923G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality IDH1 antibody (Center).
分子量	46659 Da
細胞定位	Antigen Cellular Localization: Cytoplasm. Peroxisome
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
免疫原	116-143 aa
靶點	This IDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-143 amino acids from the Central region of human IDH1.
亞型	Rabbit Ig
通用名	PICD
基因ID	3417
UniProt ID	O75874
總結(jié)	IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	IF, IHC-P, WB, FC, E
稀釋方法	IHC-P~~1:50~100 WB~~1:1000 IF~~1:10~50 FC~~1:10~50
別名	Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, IDH1, PICD
圖像	Immunohistochemical analysis of paraffin-embedded H. prostate section using IDH1 Antibody (Center)( APR07923G). APR07923G was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining. Western blot analysis of lysates from HepG2, MCF-7 cell line, human liver and rat liver tissue lysate(from left to right), using IDH1 Antibody (Center)( APR07923G). APR07923G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Confocal immunofluorescent analysis of IDH1 Antibody (Center)( APR07923G) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
說明書	Product Datasheet Protocol
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