品牌 |
Leading Biology |
貨號 |
APR08891G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
|
產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality OTC antibody (Center).
|
分子量 |
39935 Da
|
細(xì)胞定位 |
Antigen Cellular Localization:
Mitochondrion matrix.
|
宿主 |
Rabbit
|
種屬反應(yīng)性 |
Human
|
免疫原 |
71-98 aa
|
靶點(diǎn) |
This OTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-98 amino acids from the Central region of human OTC.
|
亞型 |
Rabbit Ig
|
基因ID |
|
UniProt ID |
|
總結(jié) |
OTC is a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia.
|
形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
|
儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
|
應(yīng)用 |
WB, FC, IHC-P, E
|
稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
|
別名 |
Ornithine carbamoyltransferase, mitochondrial, Ornithine transcarbamylase, OTCase, OTC
|
圖像 |
Anti-OTC Antibody (Center) at 1:1000 dilution + human liver lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 40 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
OTC Antibody (Center) (Cat. APR08891G) western blot analysis in SW620 cell line and mouse liver,rat liver lysates (35ug/lane).This demonstrates the OTC antibody detected the OTC protein (arrow).
Formalin-fixed and paraffin-embedded human hepatoarcinoma with OTC Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
|
說明書 |
|
數(shù)量 |
|