TBP Antibody (Center)

APR13719G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢(xún)價(jià)
APR13719G		100 μl / ￥4950

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TBP Antibody (Center)

品牌	Leading Biology	貨號(hào)	APR13719G
產(chǎn)品分類(lèi)	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality TBP antibody (Center).
分子量	37698 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus.
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	210-239 aa
靶點(diǎn)	This TBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-239 amino acids from the Central region of human TBP.
亞型	Rabbit Ig
通用名	GTF2D1, TF2D, TFIID
基因ID	6908
UniProt ID	P20226
功能	General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre- initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
總結(jié)	Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, FC, IF, E
稀釋方法	WB~~1:1000 IF~~1:10~50 IHC-P~~1:50~100 FC~~1:10~50
別名	TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit, TBP, GTF2D1, TF2D, TFIID
圖像	TBP Antibody (Center) (Cat. APR13719G) western blot analysis in K562,U251 cell line lysates (35ug/lane).This demonstrates the TBP antibody detected the TBP protein (arrow). Fluorescent confocal image of U251 cell stained with TBP Antibody(Center)( APR13719G).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBP primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min).TBP immunoreactivity is localized to Nucleus significantly. Formalin-fixed and paraffin-embedded human testis tissue reacted with TBP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
說(shuō)明書(shū)	Product Datasheet Protocol
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