SIRT3 Antibody (C-term)

AMM04368G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
AMM04368G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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SIRT3 Antibody (C-term)

品牌	Leading Biology	貨號	AMM04368G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SIRT3 antibody (C-term).
分子量	43573 Da
細胞定位	Antigen Cellular Localization: Mitochondrion matrix
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
免疫原	250-279 aa
靶點	This SIRT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the C-terminal region of human SIRT3.
亞型	Rabbit Ig
通用名	SIR2L3
基因ID	23410
UniProt ID	Q9NTG7
功能	NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
總結(jié)	SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100
別名	NAD-dependent protein deacetylase sirtuin-3, mitochondrial, hSIRT3, 351-, Regulatory protein SIR2 homolog 3, SIR2-like protein 3, SIRT3, SIR2L3
圖像	Anti-SIRT3 Antibody (C-term) at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-SIRT3 Antibody (C-term) at 1:2000 dilution Lane 1: human kidney lysate Lane 2: human liver lysate Lane 3: mouse liver lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of lysates from 293, HepG2 cell line (from left to right), using SIRT3 Antibody (C-term)( AMM04368G). AMM04368G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
說明書	Product Datasheet Protocol
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