ATP2A1 Antibody (C-term)

APG02093G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APG02093G		100 μl / ￥4950

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ATP2A1 Antibody (C-term)

品牌	Leading Biology	貨號	APG02093G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ATP2A1 antibody (C-term).
分子量	110252 Da
細胞定位	Antigen Cellular Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	974-1001 aa
靶點	This ATP2A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 974-1001 amino acids from the C-terminal region of human ATP2A1.
亞型	Rabbit Ig
基因ID	487
UniProt ID	O14983
功能	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
總結(jié)	ATP2A1 encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, FC, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
別名	Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, SERCA1, SR Ca(2+)-ATPase 1, Calcium pump 1, Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, ATP2A1
圖像	Western blot analysis of lysates from RD,HT1080 cell line (from left to right),using ATP2A1 Antibody (C-term)(Cat. APG02093G).APG02093G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane. ATP2A1 Antibody (C-term) (Cat. APG02093G) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP2A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. ATP2A1 Antibody (C-term) (Cat. APG02093G) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
說明書	Product Datasheet Protocol
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