Aldolase (ALDOA) Antibody (N-term)

APG01809G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APG01809G		100 μl / ￥4950

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Aldolase (ALDOA) Antibody (N-term)

品牌	Leading Biology	貨號	APG01809G
產品分類	Polyclonal Antibodies	研究領域
產品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Aldolase (ALDOA) antibody (N-term).
分子量	39420 Da
細胞定位	Antigen Cellular Localization: Cytoplasm, myofibril, sarcomere, I band. Cytoplasm, myofibril, sarcomere, M line. Note=In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca(2+)
宿主	Rabbit
種屬反應性	Human
免疫原	66-95 aa
靶點	This Aldolase (ALDOA) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-95 amino acids from the N-terminal region of human Aldolase (ALDOA).
亞型	Rabbit Ig
通用名	ALDA
基因ID	226
UniProt ID	P04075
功能	Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity).
總結	Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	IF, IHC-P, WB, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50 IF~~1:10~50
別名	Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDOA, ALDA
圖像	Western blot analysis of lysates from 293,RD,mouse NIH/3T3,rat L6 cell line (from left to right),using ALDOA Antibody (N-term)(Cat. APG01809G).APG01809G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane. Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALDOA antibody (N-term) (Cat. APG01809G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Confocal immunofluorescent analysis of Aldolase (ALDOA) Antibody (N-term)( APG01809G) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
說明書	Product Datasheet Protocol
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