ATP5I Antibody (C-term)

APG02134G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢(xún)價(jià)
APG02134G		100 μl / ￥4950

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ATP5I Antibody (C-term)

品牌	Leading Biology	貨號(hào)	APG02134G
產(chǎn)品分類(lèi)	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ATP5I antibody (C-term).
分子量	7933 Da
細(xì)胞定位	Antigen Cellular Localization: Mitochondrion. Mitochondrion inner membrane.
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
靶點(diǎn)	This ATP5I antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 55-89 amino acids from the C-terminal region of human ATP5I.
亞型	Rabbit Ig
通用名	ATP5K
基因ID	521
UniProt ID	P56385
功能	Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.
總結(jié)	Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, IF, E
稀釋方法	IF~~1:25 IHC-P~~1:25 WB~~1:1000
別名	ATP synthase subunit e, mitochondrial, ATPase subunit e, ATP5I, ATP5K
圖像	Fluorescent image of Hela cells stained with ATP5I Antibody (C-term)( APG02134G). APG02134G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red). Immunohistochemical analysis of paraffin-embedded H. liver section using ATP5I Antibody (C-term)( APG02134G). APG02134G was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining. Fluorescent image of Hela cells stained with ATP5I Antibody (C-term)( APG02134G). APG02134G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).
說(shuō)明書(shū)	Product Datasheet Protocol
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