品牌 |
Leading Biology | 貨號 |
APR08668G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality NDUFB4 antibody (N-term).
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分子量 |
15209 Da
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細胞定位 |
Antigen Cellular Localization:
Mitochondrion inner membrane; Single-pass membrane protein; Matrix side
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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靶點 |
This NDUFB4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 3-36 amino acids from the N-terminal region of human NDUFB4.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
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總結(jié) |
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, FC, IF, IHC-P, E
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稀釋方法 |
IHC-P~~1:25
IF~~1:25
FC~~1:25
WB~~1:1000
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別名 |
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, Complex I-B15, CI-B15, NADH-ubiquinone oxidoreductase B15 subunit, NDUFB4
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圖像 |
Immunohistochemical analysis of paraffin-embedded H. skin section using NDUFB4 Antibody (N-term)( APR08668G). APR08668G was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining. Fluorescent image of Hela cells stained with NDUFB4 Antibody (N-term)( APR08668G). APR08668G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red). Flow cytometric analysis of Hela cells using NDUFB4 Antibody (N-term)(green, APR08668G) compared to an isotype control of rabbit IgG(blue). APR08668G was diluted at 1:25 dilution. An Alexa Fluor? 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody. |
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說明書 |
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數(shù)量 |
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