PROX-1-S514 Antibody

APR12985G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR12985G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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PROX-1-S514 Antibody

品牌	Leading Biology	貨號	APR12985G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PROX-1-S514 antibody.
分子量	83203 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus {ECO:0000250\|UniProtKB:P48437}. Note=RORG promotes its nuclear localization {ECO:0000250\|UniProtKB:P48437}
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
免疫原	492-520 aa
靶點	This PROX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 492-520 amino acids from human PROX1.
亞型	Rabbit Ig
基因ID	5629
UniProt ID	Q92786
功能	Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.
總結(jié)	Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, FC, E
稀釋方法	FC~~1:25 WB~~1:2000 IHC-P~~1:50~100
別名	Prospero homeobox protein 1, Homeobox prospero-like protein PROX1, PROX-1, PROX1
圖像	Overlay histogram showing A549 cells stained with APR12985G (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR12985G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Anti-PROX-1-S514 Antibody at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-PROX-1-S514 Antibody at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lane 3: SW620 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說明書	Product Datasheet Protocol
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