品牌 |
Leading Biology |
貨號 |
APR15751G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality DMRTA2 antibody (C-term).
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分子量 |
53356 Da
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細胞定位 |
Antigen Cellular Localization:
Nucleus {ECO:0000255|PROSITE- ProRule:PRU00070}
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse, Zebrafish
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免疫原 |
487-515 aa
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靶點 |
This DMRTA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human DMRTA2.
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亞型 |
Rabbit Ig
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通用名 |
DMRT5
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基因ID |
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UniProt ID |
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功能 |
May be involved in sexual development.
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總結(jié) |
DMRTA2 may be involved in sexual development.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IF, E
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稀釋方法 |
WB~~1:1000
IF~~1:10~50
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別名 |
Doublesex- and mab-3-related transcription factor A2, Doublesex- and mab-3-related transcription factor 5, DMRTA2, DMRT5
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圖像 |
DMRTA2 Antibody (C-term) (Cat. APR15751G) western blot analysis in PC-3 cell line, mouse testis and zebra fish brain tissue lysates (35ug/lane).This demonstrates the DMRTA2 antibody detected the DMRTA2 protein (arrow).
Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody (C-term)( APR15751G).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.
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說明書 |
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數(shù)量 |
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