品牌 |
Leading Biology | 貨號 |
APR11729G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality DEN1A antibody (C-term).
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分子量 |
110577 Da
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細胞定位 |
Antigen Cellular Localization:
Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein. Cell junction, synapse, presynaptic cell membrane
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
576-605 aa
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靶點 |
This DEN1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 576-605 amino acids from the C-terminal region of human DEN1A.
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亞型 |
Rabbit Ig
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通用名 |
FAM31A, KIAA1608
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基因ID |
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UniProt ID |
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功能 |
Guanine nucleotide exchange factor (GEF) regulating clathrin-mediated endocytosis through RAB35 activation. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Regulates clathrin-mediated endocytosis of synaptic vesicles.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:500
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
DENN domain-containing protein 1A, Connecdenn 1, Connecdenn, Protein FAM31A, DENND1A, FAM31A, KIAA1608
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圖像 |
Anti-DEN1A Antibody (C-term) at 1:500 dilution + human liver lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 111 kDa Blocking/Dilution buffer: 5% NFDM/TBST. DEN1A Antibody (C-term) (Cat. APR11729G) IHC analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DEN1A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. DEN1A Antibody (C-term) (Cat. APR11729G) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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