品牌 |
Leading Biology | 貨號 |
APR13996G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality VTI1A antibody (C-term).
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分子量 |
25218 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasmic vesicle. Golgi apparatus membrane; Single-pass type IV membrane protein
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
140-169 aa
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靶點(diǎn) |
This VTI1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-169 amino acids from the C-terminal region of human VTI1A.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non- conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be involved in increased cytokine secretion associated with cellular senescence.
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總結(jié) |
V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. It may be concerned with increased secretion of cytokines associated with cellular senescence.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Vesicle transport through interaction with t-SNAREs homolog 1A, Vesicle transport v-SNARE protein Vti1-like 2, Vti1-rp2, VTI1A
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圖像 |
Western blot analysis of lysates from U-251 MG, U-87 MG cell line (from left to right), using VTI1A Antibody (C-term)(Cat. APR13996G). APR13996G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Formalin-fixed and paraffin-embedded human lung carcinoma with VTI1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Flow cytometric analysis of NCI-H292 cells using VTI1A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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