品牌 |
Leading Biology | 貨號(hào) |
APG02136G |
產(chǎn)品分類(lèi) |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ATP5O antibody (N-term).
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分子量 |
23277 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Mitochondrion. Mitochondrion inner membrane
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
38-64 aa
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靶點(diǎn) |
This ATP5O antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-64 amino acids from the N-terminal region of human ATP5O.
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亞型 |
Rabbit Ig
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通用名 |
ATPO
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基因ID |
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UniProt ID |
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功能 |
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
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總結(jié) |
ATP5O is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. This protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, IF, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
IF~~1:10~50
FC~~1:10~50
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別名 |
ATP synthase subunit O, mitochondrial, Oligomycin sensitivity conferral protein, OSCP, ATP5O, ATPO
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圖像 |
Western blot analysis of ATP5O Antibody (N-term) (Cat. APG02136G) in 293 cell line lysates (35ug/lane). ATP5O (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human skeletal muscle with ATP5O Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Immunofluorescence analysis of ATP5O Antibody (N-term) with paraffin-embedded human skeletal muscle. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI. |
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說(shuō)明書(shū) |
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數(shù)量 |
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