ULK3 Antibody (N-term)

AMM08431G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢(xún)價(jià)
AMM08431G		100 μl / ￥4950

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ULK3 Antibody (N-term)

品牌	Leading Biology	貨號(hào)	AMM08431G
產(chǎn)品分類(lèi)	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ULK3 antibody (N-term).
分子量	53444 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Note=Localizes to pre-autophagosomal structure during cellular senescence
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
免疫原	14-43 aa
靶點(diǎn)	This ULK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-43 amino acids from the N-terminal region of human ULK3.
亞型	Rabbit Ig
基因ID	25989
UniProt ID	Q6PHR2
功能	Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.
總結(jié)	ULK3 is a member of the ULK kinase family, one of three human homologs of the yeast autophagy-specific kinase.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50
別名	Serine/threonine-protein kinase ULK3, Unc-51-like kinase 3, ULK3
圖像	Anti-ULK3 Antibody (N-term) at 1:2000 dilution + LNCaP whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of anti-ULK3 Antibody (N-term) (Cat. AMM08431G) in mouse heart tissue lysates (35ug/lane).ULK3(arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human testis tissue reacted with *ULK3 antibody (N-term) (Cat. AMM08431G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
說(shuō)明書(shū)	Product Datasheet Protocol
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