EN1 (Engrailed 1) Antibody (N-term)

APR11818G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR11818G		100 μl / ￥4950

大規(guī)格詢價

3 Images

品牌	Leading Biology	貨號	APR11818G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality EN1 (Engrailed 1) antibody (N-term).
分子量	40115 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus.
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	1-30 aa
靶點(diǎn)	This EN1 (Engrailed 1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EN1 (Engrailed 1).
亞型	Rabbit Ig
基因ID	2019
UniProt ID	Q05925
總結(jié)	Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, IF, E
稀釋方法	WB~~1:1000 IF~~1:10~50 IHC-P~~1:10~50
別名	Homeobox protein engrailed-1, Homeobox protein en-1, Hu-En-1, EN1
圖像	All lanes : Anti-EN1 Antibody (N-term) at 1:1000 dilution Lane 1: human fetal brain lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS (Human Sarcoma cell line) cells labeling Pdx1 with APR11818G at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Immunofluorescence image showing nucleus and nucleoli staining on U-2 OS cell line. Cytoplasmic actin is detected with Alexa Fluor? 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red). APR11818G staining EN1 in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
說明書	Product Datasheet Protocol
數(shù)量

選擇	品牌	貨號	產(chǎn)品名稱	規(guī)格	分類	研究領(lǐng)域	說明書	數(shù)量	目錄價
1	Leading Biology	APR05963G	EGFR Antibody (Y1092)	100 μl	Polyclonal Antibodies				￥4950.00	訂購詢價
2	Leading Biology	APR03116G	BRCA1 Antibody (aa1847-1863)	100 μl	Polyclonal Antibodies				￥4950.00	訂購詢價
3	Leading Biology	AMM05633G	Bombesin Receptor 2 (extracellular) Antibody	50 μl	Polyclonal Antibodies				￥6950.00	訂購詢價
4	Leading Biology	APR10986G	AP3B1 Antibody (aa2-14)	50 μg	Polyclonal Antibodies				￥4950.00	訂購詢價
5	Leading Biology	APR11512G	Bestrophin-1 (extracellular) Antibody	50 μl	Polyclonal Antibodies				￥6950.00	訂購詢價
6	Leading Biology	APG01407G	A3 Adenosine Receptor Antibody	50 μl	Polyclonal Antibodies				￥6950.00	訂購詢價

最新加入的商品

最新加入的商品

全部產(chǎn)品分類

EN1 (Engrailed 1) Antibody (N-term)

EN1 (Engrailed 1) Antibody (N-term)

熱銷產(chǎn)品

相關(guān)產(chǎn)品

關(guān)注安諾倫

安諾倫官方微信

產(chǎn)品添加成功！

加入詢價單成功！

最新加入的商品

最新加入的商品

全部產(chǎn)品分類

EN1 (Engrailed 1) Antibody (N-term)

EN1 (Engrailed 1) Antibody (N-term)

熱銷產(chǎn)品

相關(guān)產(chǎn)品

關(guān)注安諾倫

安諾倫官方微信

產(chǎn)品添加成功！

加入詢價單成功！

加入詢價單成功！