品牌 |
Leading Biology | 貨號(hào) |
AMM04633G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality FUCA1 antibody (Center).
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分子量 |
53689 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Lysosome.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
265-294 aa
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靶點(diǎn) |
This FUCA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the Central region of human FUCA1.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N- acetylglucosamine of the carbohydrate moieties of glycoproteins.
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總結(jié) |
FUCA1 is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids. Mutations in this gene are associated with fucosidosis (FUCA1D), which is an autosomal recessive lysosomal storage disease. A pseudogene of this locus is present on chr 2.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Tissue alpha-L-fucosidase, Alpha-L-fucosidase I, Alpha-L-fucoside fucohydrolase 1, Alpha-L-fucosidase 1, FUCA1
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圖像 |
Western blot analysis of FUCA1 Antibody (Center) (Cat. AMM04633G) in HepG2 cell line lysates (35ug/lane). FUCA1 (arrow) was detected using the purified Pab. FUCA1 Antibody (Center) (Cat. AMM04633G) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FUCA1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. FUCA1 Antibody (Center) (Cat. AMM04633G) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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