品牌 |
Leading Biology | 貨號 |
APG01862G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality AMT antibody (N-term).
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分子量 |
43946 Da
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細胞定位 |
Antigen Cellular Localization:
Mitochondrion.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
19-45 aa
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靶點 |
This AMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-45 amino acids from the N-terminal region of human AMT.
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亞型 |
Rabbit Ig
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通用名 |
GCST
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基因ID |
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UniProt ID |
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功能 |
The glycine cleavage system catalyzes the degradation of glycine.
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總結(jié) |
The enzyme system for cleavage of glycine (glycine cleavage system; EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase; MIM 238300), H protein (a lipoic acid-containing protein; MIM 238330), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase; MIM 238331). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Aminomethyltransferase, mitochondrial, Glycine cleavage system T protein, GCVT, AMT, GCST
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圖像 |
Western blot analysis of AMT Antibody (N-term) (Cat. APG01862G) in HepG2 cell line lysates (35ug/lane). AMT (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with AMT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. AMT Antibody (N-term) (Cat. APG01862G) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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