品牌 |
Leading Biology | 貨號 |
AMM07666G |
產品分類 |
Polyclonal Antibodies | 研究領域 |
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產品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality RRAGD antibody (Center).
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分子量 |
45588 Da
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細胞定位 |
Antigen Cellular Localization:
Cytoplasm. Nucleus. Lysosome. Note=Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA
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宿主 |
Rabbit
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種屬反應性 |
Human, Mouse
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免疫原 |
171-199 aa
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靶點 |
This RRAGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-199 amino acids from the Central region of human RRAGD.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.
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總結 |
RRAGD is a monomeric guanine nucleotide-binding protein, or G protein. By binding GTP or GDP, small G proteins act as molecular switches in numerous cell processes and signaling pathways.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Ras-related GTP-binding protein D, Rag D, RagD, RRAGD (HGNC:19903)
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圖像 |
Anti-RRAGD Antibody (Center) at 1:1000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of RRAGD Antibody (Center) (Cat. AMM07666G) in MDA-MB435 cell line lysates (35ug/lane). RRAGD (arrow) was detected using the purified Pab. Western blot analysis of RRAGD Antibody (Center) (Cat. AMM07666G) in mouse cerebellum tissue lysates (35ug/lane). RRAGD (arrow) was detected using the purified Pab. |
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說明書 |
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數量 |
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