品牌 |
Leading Biology |
貨號 |
AMM07275G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PPIA antibody (N-term).
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分子量 |
18012 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm. Secreted. Note=Secretion occurs in response to oxidative stress in vascular smooth muscle through a vesicular secretory pathway that involves actin remodeling and myosin II activation, and mediates ERK1/2 activation
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse
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免疫原 |
8-37 aa
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靶點(diǎn) |
This PPIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-37 amino acids from the N-terminal region of human PPIA.
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亞型 |
Rabbit Ig
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通用名 |
CYPA
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基因ID |
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UniProt ID |
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功能 |
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
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總結(jié) |
PPIA is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
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別名 |
Peptidyl-prolyl cis-trans isomerase A, PPIase A, Cyclophilin A, Cyclosporin A-binding protein, Rotamase A, Peptidyl-prolyl cis-trans isomerase A, N-terminally processed, PPIA, CYPA
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圖像 |
All lanes : Anti-PPIA Antibody (N-term) at 1:1000 dilution
Lane 1: A431 whole cell lysate
Lane 2: Hela whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 18 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of PPIA Antibody (N-term) (Cat. AMM07275G) in T47D cell line(lane 1) and mouse brain(lane 2),bladder(lane 3) tissue lysates (35ug/lane). PPIA (arrow) was detected using the purified Pab.(2ug/ml)
Formalin-fixed and paraffin-embedded human brain tissue reacted with PPIA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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說明書 |
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數(shù)量 |
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