品牌 |
Leading Biology | 貨號 |
APR09113G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PHGDH antibody (Center).
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分子量 |
56651 Da
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
249-277 aa
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靶點(diǎn) |
This PHGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-277 amino acids from the Central region of human PHGDH.
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亞型 |
Rabbit Ig
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通用名 |
PGDH3
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基因ID |
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UniProt ID |
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總結(jié) |
3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95)catalyzes the transition of 3-phosphoglycerate into3-phosphohydroxypyruvate, which is the first and rate-limiting stepin the phosphorylated pathway of serine biosynthesis, usingNAD+/NADH as a cofactor.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
IHC-P~~1:50~100
FC~~1:10~50
WB~~1:1000
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別名 |
D-3-phosphoglycerate dehydrogenase, 3-PGDH, PHGDH, PGDH3
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圖像 |
PHGDH Antibody (Center) (Cat. APR09113G) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PHGDH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. PHGDH Antibody (Center) (Cat. APR09113G) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. PHGDH Antibody (Center) (Cat. APR09113G) western blot analysis in Ramos cell line lysates (15ug/lane).This demonstrates the PHGDH antibody detected the PHGDH protein (arrow). |
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說明書 |
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數(shù)量 |
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