品牌 |
Leading Biology |
貨號(hào) |
APG02192G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality BARON antibody (Center).
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分子量 |
108622 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Late endosome. Lysosome. Early endosome. Note=Predominantly located in late endosomes/lysosomes, only partially detected in early endososome and not at all in the Golgi apparatus
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
656-684 aa
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靶點(diǎn) |
This BARON antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656-684 amino acids from the Central region of human BARON.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Negatively regulates endocytic trafficking. Impairs the autophagosome maturation process. May inhibit PIK3C3 activity (By similarity).
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
Run domain Beclin-1 interacting and cysteine-rich containing protein, Rubicon, Beclin-1 associated RUN domain containing protein, Baron, KIAA0226
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圖像 |
All lanes : Anti-BARON Antibody (Center) at 1:1000 dilution
Lane 1: MCF-7 whole cell lysate
Lane 2: Raji whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 109 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human kidney reacted with BARON Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
BARON Antibody (Center) (Cat. APG02192G) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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說(shuō)明書(shū) |
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數(shù)量 |
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