品牌 |
Leading Biology |
貨號(hào) |
APR07647G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality EHD1 antibody (C-term).
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分子量 |
60627 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Recycling endosome membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Preferentially associates with tubular recycling endosomes. Colocalizes with FER1L5 at plasma membrane in myoblasts and myotubes
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
410-438 aa
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靶點(diǎn) |
This EHD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-438 amino acids from the C-terminal region of human EHD1.
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亞型 |
Rabbit Ig
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通用名 |
PAST, PAST1
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基因ID |
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UniProt ID |
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功能 |
Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Plays a role in myoblast fusion. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth.
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總結(jié) |
This gene belongs to a highly conserved gene familyencoding EPS15 homology (EH) domain-containing proteins. Theprotein-binding EH domain was first noted in EPS15, a substrate forthe epidermal growth factor receptor. The EH domain has been shownto be an important motif in proteins involved in protein-proteininteractions and in intracellular sorting. The protein encoded bythis gene is thought to play a role in the endocytosis of IGF1receptors.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, E
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稀釋方法 |
WB~~1:1000
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別名 |
EH domain-containing protein 1, PAST homolog 1, hPAST1, Testilin, EHD1, PAST, PAST1
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圖像 |
All lanes : Anti-EHD1 Antibody (C-term) at 1:1000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: Hela whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 61kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
EHD1 Antibody (C-term) (Cat. APR07647G) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the EHD1 antibody detected the EHD1 protein (arrow).
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說明書 |
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數(shù)量 |
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