RHBDF2 Antibody (N-term)

AMM07604G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
AMM07604G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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RHBDF2 Antibody (N-term)

品牌	Leading Biology	貨號	AMM07604G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RHBDF2 antibody (N-term).
分子量	96686 Da
細(xì)胞定位	Antigen Cellular Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	80-109 aa
靶點	This RHBDF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-109 amino acids from the N-terminal region of human RHBDF2.
亞型	Rabbit Ig
通用名	IRHOM2, RHBDL5, RHBDL6
基因ID	79651
UniProt ID	Q6PJF5
功能	Rhomboid protease-like protein which has no protease activity but regulates the secretion of several ligands of the epidermal growth factor receptor. Indirectly activates the epidermal growth factor receptor signaling pathway and may thereby regulate sleep, cell survival, proliferation and migration (By similarity).
總結(jié)	Not expected to have protease activity (By similarity).
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	IHC-P, WB, E
稀釋方法	IHC-P~~1:25 WB~~1:1000
別名	Inactive rhomboid protein 2, iRhom2, Rhomboid 5 homolog 2, Rhomboid family member 2, Rhomboid veinlet-like protein 5, Rhomboid veinlet-like protein 6, RHBDF2, IRHOM2, RHBDL5, RHBDL6
圖像	AMM07604G staining RHBDF2 in human duodenum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. AMM07604G staining RHBDF2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-RHBDF2 Antibody (N-term) at 1:2000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說明書	Product Datasheet Protocol
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