品牌 |
Leading Biology | 貨號 |
APR08489G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality mouse BID antibody (S61).
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分子量 |
21952 Da
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細胞定位 |
Antigen Cellular Localization:
Cytoplasm. Mitochondrion membrane. Note=When uncleaved, it is predominantly cytoplasmic. p15 BID translocates to mitochondria as an integral membrane protein. p13 and p22 BID are associated with the mitochondrial membrane
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宿主 |
Rabbit
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種屬反應性 |
Mouse
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免疫原 |
39-68 aa
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靶點 |
This mouse BID antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from mouse BID.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Induces caspases and apoptosis. Counters the protective effect of Bcl-2. The major proteolytic product p15 BID allows the release of cytochrome c.
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總結(jié) |
Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
BH3-interacting domain death agonist, p22 BID, BID, BH3-interacting domain death agonist p15, p15 BID, BH3-interacting domain death agonist p13, p13 BID, BH3-interacting domain death agonist p11, p11 BID, Bid
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圖像 |
Western blot analysis of anti-mouse BID Antibody (S61) (Cat. APR08489G) in mouse spleen tissue lysates (35ug/lane). BID (arrow) was detected using the purified Pab . mouse BID Antibody (S61)(Cat. APR08489G) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the mouse BID Antibody (S61) for immunohistochemistry. Clinical relevance has not been evaluated. mouse BID Antibody (S61) (Cat. APR08489G) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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