GSTM5 Antibody (N-term)

AMM05256G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價(jià)格	詢價(jià)
AMM05256G		100 μl / ￥4950

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GSTM5 Antibody (N-term)

品牌	Leading Biology	貨號	AMM05256G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GSTM5 antibody (N-term).
分子量	25675 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm.
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	14-43 aa
靶點(diǎn)	This GSTM5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-43 amino acids from the N-terminal region of human GSTM5.
亞型	Rabbit Ig
基因ID	2949
UniProt ID	P46439
功能	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
總結(jié)	Cytosolic and membrane-bound forms of glutathioneS-transferase are encoded by two distinct supergene families. Atpresent, eight distinct classes of the soluble cytoplasmicmammalian glutathione S-transferases have been identified: alpha,kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes aglutathione S-transferase that belongs to the mu class. The muclass of enzymes functions in the detoxification of electrophiliccompounds, including carcinogens, therapeutic drugs, environmentaltoxins and products of oxidative stress, by conjugation withglutathione. The genes encoding the mu class of enzymes areorganized in a gene cluster on chromosome 1p13.3 and are known tobe highly polymorphic. These genetic variations can change anindividual's susceptibility to carcinogens and toxins as well asaffect the toxicity and efficacy of certain drugs. Diversificationof these genes has occurred in regions encoding substrate-bindingdomains, as well as in tissue expression patterns, to accommodatean increasing number of foreign compounds.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, FC, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50
別名	Glutathione S-transferase Mu 5, GST class-mu 5, GSTM5-5, GSTM5
圖像	Western blot analysis of lysate from human brain tissue lysate, using GSTM5 Antibody (N-term)(Cat. AMM05256G). AMM05256G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. GSTM5 Antibody (N-term) (Cat. AMM05256G)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GSTM5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. GSTM5 Antibody (N-term) (Cat. AMM05256G) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
說明書	Product Datasheet Protocol
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