品牌 |
Leading Biology | 貨號 |
APR08965G |
產品分類 |
Polyclonal Antibodies | 研究領域 |
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產品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PCCA antibody (Center).
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分子量 |
80059 Da
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細胞定位 |
Antigen Cellular Localization:
Mitochondrion matrix
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宿主 |
Rabbit
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種屬反應性 |
Human, Rat
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免疫原 |
362-390 aa
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靶點 |
This PCCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 362-390 amino acids from the Central region of human PCCA.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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總結 |
The protein encoded by this gene is the alpha subunit ofthe heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase.PCCA encodes the biotin-binding region of this enzyme. Mutations ineither PCCA or PCCB (encoding the beta subunit) lead to an enzymedeficiency resulting in propionic acidemia. Multiple transcriptvariants encoding different isoforms have been found for this gene.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
IHC-P, WB, E
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稀釋方法 |
IHC-P~~1:25
WB~~1:1000
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別名 |
Propionyl-CoA carboxylase alpha chain, mitochondrial, PCCase subunit alpha, Propanoyl-CoA:carbon dioxide ligase subunit alpha, PCCA
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圖像 |
APR08965G staining PCCA in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. APR08965G staining PCCA in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. APR08965G staining PCCA in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. |
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說明書 |
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數量 |
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