SP1 Antibody (C-term P692)

APR07185G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR07185G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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Tel: 010-56256916
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SP1 Antibody (C-term P692)

品牌	Leading Biology	貨號(hào)	APR07185G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SP1 antibody (C-term P692).
分子量	80693 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus. Cytoplasm. Note=Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	677-707 aa
靶點(diǎn)	This SP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 677-707 amino acids from the C-terminal region of human SP1.
亞型	Rabbit Ig
通用名	TSFP1
基因ID	6667
UniProt ID	P08047
功能	Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR- alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component ARNTL/BMAL1.
總結(jié)	Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, FC, IF, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 IF~~1:10~50
別名	Transcription factor Sp1, SP1, TSFP1
圖像	Anti-SP1 Antibody (C-term P692) at 1:500 dilution + Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 81 kDa Blocking/Dilution buffer: 5% NFDM/TBST. SP1 Antibody (C-term P692) (Cat. APR07185G) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SP1 antibody detected the SP1 protein (arrow). SP1 Antibody (C-term P692) (Cat. APR07185G)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SP1 Antibody (C-term P692) for immunohistochemistry. Clinical relevance has not been evaluated.
說(shuō)明書	Product Datasheet Protocol
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