CTLA4 Antibody (N-term)

APR15622G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR15622G		100 μl / ￥4950

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品牌	Leading Biology	貨號	APR15622G
產(chǎn)品分類	Polyclonal Antibodies	研究領域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CTLA4 antibody (N-term).
分子量	24656 Da
細胞定位	Antigen Cellular Localization: Cell membrane; Single-pass type I membrane protein Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation;
宿主	Rabbit
種屬反應性	Human
免疫原	50-78 aa
靶點	This IDDM12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-78 amino acids from the N-terminal region of human IDDM12.
亞型	Rabbit Ig
通用名	CD152
基因ID	1493
UniProt ID	P16410
功能	Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
總結	CTLA4 is a member of the immunoglobulin superfamilyand encodes a protein which transmits an inhibitory signal to Tcells. The protein contains a V domain, a transmembrane domain, anda cytoplasmic tail. Alternate transcriptional splice variants,encoding different isoforms, have been characterized. Themembrane-bound isoform functions as a homodimer interconnected by adisulfide bond, while the soluble isoform functions as a monomer.Mutations in this gene have been associated with insulin-dependentdiabetes mellitus, Graves disease, Hashimoto thyroiditis, celiacdisease, systemic lupus erythematosus, thyroid-associatedorbitopathy, and other autoimmune diseases.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	WB, IHC-P, E
稀釋方法	WB~~1:2000 IHC-P~~1:50~100
別名	Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CTLA4, CD152
圖像	All lanes : Anti-CTLA4 Antibody (N-term) at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-CTLA4 Antibody (N-term) at 1:2000 dilution Lane 1: WiDr whole cell lysates Lane 2: Raji whole cell lysates Lane 3: K562 whole cell lysates Lane 4: Jurkat whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST. CTLA4 Antibody (N-term) (Cat. AP10465a) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CTLA4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
說明書	Product Datasheet Protocol
數(shù)量

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