UBA52 Antibody (C-Term)

APR13896G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR13896G		100 μl / ￥4950

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UBA52 Antibody (C-Term)

品牌	Leading Biology	貨號(hào)	APR13896G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality UBA52 antibody (C-Term).
分子量	14728 Da
細(xì)胞定位	Antigen Cellular Localization: Ubiquitin: Cytoplasm. Nucleus
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	100-128 aa
靶點(diǎn)	This UBA52 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-128 amino acids from the C-terminal region of human UBA52.
亞型	Rabbit Ig
通用名	UBCEP2
基因ID	7311
UniProt ID	P62987
功能	Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
總結(jié)	Ubiquitin is a highly conserved nuclear and cytoplasmicprotein that has a major role in targeting cellular proteins fordegradation by the 26S proteosome. It is also involved in themaintenance of chromatin structure, the regulation of geneexpression, and the stress response. Ubiquitin is synthesized as aprecursor protein consisting of either polyubiquitin chains or asingle ubiquitin moiety fused to an unrelated protein. This geneencodes a fusion protein consisting of ubiquitin at the N terminusand ribosomal protein L40 at the C terminus, a C-terminal extensionprotein (CEP). Multiple processed pseudogenes derived from thisgene are present in the genome.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, FC, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
別名	Ubiquitin-60S ribosomal protein L40, CEP52, Ubiquitin A-52 residue ribosomal protein fusion product 1, Ubiquitin, 60S ribosomal protein L40, UBA52, UBCEP2
圖像	UBA52 Antibody (C-Term) (Cat. APR13896G) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UBA52 antibody detected the UBA52 protein (arrow). UBA52 antibody (C-Term) (Cat. APR13896G) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the UBA52 antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated. UBA52 Antibody (C-Term) (Cat. APR13896G) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
說明書	Product Datasheet Protocol
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