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Phospho-Ser150 Troponin I (cardiac) Antibody品牌 |
Leading Biology | 貨號(hào) |
APR03401G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Phospho-Ser150 Troponin I (cardiac) Antibody.
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分子量 |
25 KDa
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Mouse, Rat
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靶點(diǎn) |
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser150 conjugated to KLH.
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基因ID |
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UniProt ID |
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總結(jié) |
Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser23/24 (Noland et al, 1995) and is phosphorylated by AMPK at Ser23 and Ser150 (Solis et al, 2011). Evidence suggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser150 over Ser23, and may play a role in regulating energy consumption through altering the phosphorylation status of cTnI (Solis et al., 2011).
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB
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稀釋方法 |
WB~~ 1:1000
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圖像 |
Western blot of mouse heart lysate showing specific immunolabeling of ~25k cTnI protein phosphorylated at Ser150 (control). Phosphospecificity is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that the lysate was incubated in λ-Ptase (1400 units for 30 min). The immunolabeling is greatly decreased by treatment with λ-Ptase. |
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說明書 |
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數(shù)量 |
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